深圳地区麻疹病毒核蛋白N基因序列分析

Sequence analysis on nucleopotein(N) gene of mealses virus isolated in Shenzhen

摘要:
目的探讨深圳市麻疹病毒的基因型别和特征。
方法采用B95a细胞分离培养麻疹病毒, 通过RT-PCR方法扩增麻疹病毒核蛋白N基因C末端450bp片段并测序, 分析和基因库Genebank中麻疹病毒的各基因型代表株序列的同源性。
结果分离到1株麻疹病毒, 与H₁基因型代表株相比同源性为98.4%。
结论深圳市麻疹流行株属于H₁基因型。

Abstract:
Objective To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen during the year of 2003.
Methods Throat swabs were collected for virus isolation.A 450 bp fragment of the C-terminal of the nucleoprotein (N) gene was amplified by RT-PCR and subjected to sequence and phylog enetic analysis using DANSTAR software.
Results Only one strain was isolated from 15 throat-swabs. N genes homog enetity was 98.4% compared with other wild-type viruses of H₁ genotype.
Conclusion Shenzhen circulating measles viruses were belonged to the clade H₁.