三氧化二砷对黄鳝外周血红细胞微核的影响
Effects of As2O3 on micronucleus in peripheral blood erythrocytes of monopterus albus
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摘要:目的 观察不同浓度的三氧化二砷对黄鳝外周血红细胞微核和核异常的影响。方法 采用鱼类致突变实验方法, 以0.25, 0.50, 0.75, 100, 150mg/kg不同剂量三氧化二砷处理黄鳝, 测定黄鳝外周血红细胞微核率和核异常率、过氧化氢酶活性。结果 0.50mg/kg剂量组的微核率与核异常率均高于0.25mg/kg剂量组。0.75mg/kg剂量组的微核率与核异常率均下降, 且低于阴性对照组, 但无显著性差异(P > 0.05)。剂量继续增加到100, 150mg/kg时, 其核异常率又显著回升, 而微核率回升却并不显著。0.25~100mg/kg4个剂量均使过氧化氢酶(CAT)活性增加。结论 三氧化二砷对黄鳝外周血红细胞的作用具有双向性, 且对CAT具有激活作用。Abstract:Objective To study the influence of the different concentration of As2O3 on micronuclei and nuclear abnormity in peripheral blood erythrocytes of monoopterus albus.Methods The test of fish mutagenesis is used to assay the rate of micronucleus and nuclear anomaly in peripheral blood erythro cytes, and CAT activity of monopterus albus as mutagen of 0.25, 0.50, 0.75, 1.00 and 1.50 mg/kg different concentration of As2O3.Results The rate of micronuclei and the rate of nuclear abnormity at 0.50 mg/kg are higher than that at 0.25 mg/kg.When the dosage is 0.75 mg/kg, the rate of nuclear anomalies is lower than that of the negative group, but the difference is not marked.The rate of nuclear abnormity r ises markedly and the rate of micronuclei doesn't rise markedly at the dosage of 1.00 and 1.50 mg/kg.The 4 doses of As2O3 from 0.25 As2O3 to 1.00 mg/kg have an activation effect on CAT.Conclusion The effect of As2O3 has two sides on peripheral blood erythrocytes of monopterus albus.As2O3 has an activation effect on CAT.
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