鞘氨醇对人结肠癌细胞的抑制作用

宋士利; 李百祥; 韩云峰

鞘氨醇对人结肠癌细胞的抑制作用

Inhibitory effect of sphingosine on human colon HT-29 cells

摘要

摘要:
目的研究鞘氨醇对人结肠癌细胞的影响。
方法采用四甲基偶氮唑盐法(MTT)、细胞核分裂指数和³H-TdR掺入试验方法, 所设剂量鞘氨醇分别为0, 0.82, 1.63, 3.125, 6.25, 12.5μmol/L, 以二甲基亚砜(DMSO)为溶剂对照。
结果鞘氨醇对HT-29细胞的生长有明显抑制作用。在MTT试验中, 不同浓度鞘氨醇对HT-29细胞的7d抑制率分别为18%, 45%, 72%, 87%和89%;在核分裂指数试验中, 随着鞘氨醇剂量的增高, 其核分裂指数明显降低; 在³H-TdR掺入试验中, 可见随着鞘氨醇剂量的增高, ³H-TdR掺入到HT-29细胞中明显的减少, 与阴性对照组相比有显著性差异。
结论鞘氨醇对HT-29细胞的生长增殖有明显抑制作用, 这可能是鞘磷脂抑制结肠癌的机制之一。

Abstract:
Objective To study the influence of sphingosine on the growth of human colon HT-29 cells in vitro.
Methods Using cell culture tests (MTT test, index of cellular mitosis, and ³H-TdR incorporation test), to examine the growth of HT-29 cells at five concentrations (0, 0.82, 1.63, 3.125, 6.25, 12.5μmol/L)with a negative control (DMSO v/v).
Results Sphingosine can inhibited the growth of HT-29 cells. After treatment with various sophingosine concentrations mentioned above for 7 days, the inhibition rates were 18%, 45%, 72%, 87% and 89% respectively. With the increase of sphingosine concentrations, ³H-TdR incorporation was decreased in HT-29 cells and the quantity of ³H-TdR incorporation was significantly decreased as compared with the negative control (P < 0.05).
Conclusion Sphingosine can significantly inhibit the growth of HT-29 cells, which was probably one of the preventive mechanisms of sphingomyelin for colon cancer.

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