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刘运喜, 高媛, 赵仲堂, 张景兰, 杨占清, 步秀萍, 苏静静. 秋冬型恙虫病病人、恙螨、鼠东方体基因型研究[J]. 中国公共卫生, 2004, 20(7): 780-782.
引用本文: 刘运喜, 高媛, 赵仲堂, 张景兰, 杨占清, 步秀萍, 苏静静. 秋冬型恙虫病病人、恙螨、鼠东方体基因型研究[J]. 中国公共卫生, 2004, 20(7): 780-782.
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LIU Yun-xi, GAO Yuan, ZHAO Zhong-tang, . Study on gene homology of orientia tsutugamushi isolated from scrub typhus patients, chigger mites, and rodents[J]. Chinese Journal of Public Health, 2004, 20(7): 780-782.
Citation: LIU Yun-xi, GAO Yuan, ZHAO Zhong-tang, . Study on gene homology of orientia tsutugamushi isolated from scrub typhus patients, chigger mites, and rodents[J]. Chinese Journal of Public Health, 2004, 20(7): 780-782.
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秋冬型恙虫病病人、恙螨、鼠东方体基因型研究

Study on gene homology of orientia tsutugamushi isolated from scrub typhus patients, chigger mites, and rodents

    摘要
  • 摘要:
      目的   了解秋冬型恙虫病流行季节黑线姬鼠、小盾纤恙螨、病人体内恙虫病东方体(Orientiatsut sugamushi, Ot)基因型的一致性。
      方法   采用NestedPCR结合限制性内切酶片段长度多态分析(RFLP)技术对山东地区恙虫病流行季节病人、野外优势鼠种黑线姬鼠、鼠体外优势螨种小盾纤恙螨标本中Ot-Sta56基因型进行分析。
      结果   应用PCR/RFLP分析了12株分离自山东秋冬型恙虫病流行季节病人、黑线姬鼠、小盾纤恙螨体内的Ot, 2份采自黑线姬鼠体外的小盾纤恙螨匀浆及10份恙虫病人全血中的Ot-sta56目的基因, 24份标本酶切图谱经与已知序列的Ot内切酶图谱相比, 与日本地方株-Kawasaki株具有相似的酶切图谱, 但缺乏HhaI的酶切位点; 24份标本提取的Ot-DNA扩增产物分别经型引物扩增, 均出现相应分子量的扩增片段, 皆属日本地方株Kawasaki型。
      结论   秋冬型恙虫病流行季节病人、野外优势鼠种黑线姬鼠、鼠体外优势螨种小盾纤恙螨体内所分离的恙虫病东方体Sta56基因型是一致的, 并在病人、黑线姬鼠、小盾纤恙螨间构成相互传播关系, 这对当地恙虫病疫源地的维持有重要作用。

     

    Abstract:
      Objective   In order to detect the gene homologies of orientia tsutsugamushi (Ot) isolated from scrub typhus patients, chigger mites, and rodents of Shandong province in epidemic season.
      Methods   Nested-PCR connected with PCR/RFLP was used to identify the gene type of 12 strains Ot isolated from scrub typhus patients, the dominant chigger mite Leptotrombidium (L.) scutellare, and the dominant rodent Apodemus (A) agrarius in epidemic season of scrub typhus, 2 pools of homogenized L. scutellare, and 10 blood of scrub typhus, and compared with international reference strains Gilliam、Karp、Kato.
      Results   Using PCR/RFLP, 24 had the similar restriction maps with Japan Kawasaki strain, but they had not restriction site of Hha Ⅰ, and thus had some difference in gene sequence compared with Japan Kawasaki strain. Using Nested-PCR, 24 had the same products in the amplification of template Ot-DNA, thus they all belonged to Kawasaki strains, which was endemic in Japan.
      Conclusion   The gene types of Ot isolated from scrubty phuspatients, chigger mites, and rodents of Shandong province in epidemic season were identical, they all were similar to Japan Kawasaki strain, buthad some differences in gene sequence, and the transmission of Ot among patients, chigger mites, and rodents existed in Shandong province.

     

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