氧化型低密度脂蛋白诱导内皮细胞基因表达
Study on early responses of vascular endothelial cells induced by oxidized low density lipoprotein
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摘要:目的 通过研究氧化型低密度脂蛋白(Oxidized low density lipoprotein, Ox-LDL)诱导血管内皮细胞基因表达谱的改变, 为阐明氧化型低密度脂蛋白致内皮细胞功能障碍及动脉粥样硬化形成的分子机制提供科学依据。方法 采用含有4000条全长已知人类基因cDNA, 以及96条参照基因cDNA克隆制作的基因表达谱芯片, 筛查氧化型低密度脂蛋白(100mg/L)作用6h对人脐静脉血管内皮细胞的基因表达谱改变的影响。结果 Ox-LDL可诱导3条基因表达下调, 5条基因表达上调。结论 氧化型低密度脂蛋白的早期作用即可诱导内皮细胞相关基因的表达改变; 首次发现氧化型低密度脂蛋白可诱导内皮细胞H731蛋白, ST2蛋白, CyclinB1(细胞周期蛋白B1)和KDRF(KM-102-derive dreductase-like factor, KM-102源性还原酶样因子)等基因表达的改变, 为揭示氧化型低密度脂蛋白引起血管内皮细胞功能障碍的机制提供线索。Abstract:Objective To study the change of the gene expression pattern of vascular endothelial cells induced by oxidized low density lipoprotein.Methods After human umbilical vein endothelial cells was exposed to oxidized low density lipoprotein(100 mg/L)for 6 hr, genes expression was screened with a complementary DNA microarray representing 4 000 different human genes and 96 control genes.Results There were 3 down-regulated gene and 5 up-regulated genes.Conclusion Ox-LDL could induce the change of gene expression pattern of endothelial cells. It was the first time to find that Ox-LDL could induce the change of the gene expression of H731, ST2 protein, Cyclin B1 and KDRF. These changes might be related to the dysfunction of endothelial cells induced by Ox-LDL.
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