构树叶总黄酮对人永生化表皮细胞的防护效果

杨雪莹; 何瑞; 王亭; 曹玉广

构树叶总黄酮对人永生化表皮细胞的防护效果

Protective effects of TFBP on human immortalized keratinocytes line HaCaT

摘要

摘要:
目的探讨构树叶总黄酮(total flzvonoids of Broussonetia papyrifera, TFBP)对铅、砷引起的人永生化表皮细胞活性损伤和氧化损伤的防护效果。
方法采集构树叶并利用聚酰胺柱洗脱法提取构树叶总黄酮。用不同剂量的铅和砷处理细胞, 加入不同浓度的构树叶总黄酮, 采用噻唑蓝(MTT)染色法检测细胞活性变化, 采用丙二醛(MDA)试剂盒测MDA的变化。
结果 0.1~1.0mmol/L的醋酸铅对细胞的活性有抑制作用。低浓度亚砷酸钠(0.5~1.0μmol/L)对人永生化表皮细胞有增值作用, 高于5.0μmol/L的亚砷酸钠对表皮细胞生长有抑制作用。添加大于10.0mg/LTFBP后与对照组比较, MTT值明显升高, MDA的含量明显降低, 有显著性差异。
结论醋酸铅和亚砷酸钠对人永生化表皮细胞有活性损伤和氧化损伤, 构树叶总黄酮TFBP能降低这种氧化损伤, 对细胞有防护功能。

Abstract:
Objective To investigate the damage of lead acetate and sodium arsenite on human immotalized keratinocytes line HaCaT and the protective effects of TFBP(total flzvonoids of broussonetia papyrifera)gotten from the leaves of broussoneria papyifera.
Methods TFBP were gotten by use of the absorption chromatograph column of polyamide. MTT reduction assay were performed and the levels of malondialdehyde (MDA)were determined in cultured HaCaT cells. Human immotlaized keratinocytes HaCaT cells were irradiated by lead acetate and sodium arsenite radiation at different dosages. The protective effects of TFBP also were evaluated.
Results With the different concentrations of 0.1-1.0 mmol/L lead acetate, the skin cells were markedly inhibited. Sodium arsenite at the lower concentrations of 0.5-1.0μmol/L induced mainly the degree of the proliferation of the HaCaT cells, while inhibiting cell growth over 5μmol/L.TFBP at the higher concentrations of 100 mg/L had certain protective effects from lead acetate or sodium arsenite, and the levels of MDA decreased, too.
Conclusion Lead acetate and sodium arsenite could cause significant damage to HaCaT cells under the conditions of this study, TFBP gotten from the leaves of broussoneria papyifera had certain protective effects on HaCaT epithelial cells from those damage.

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