氟对大鼠腰椎松质骨超微结构的影响
Scanning electron microscopic analysis on lumbar vertebrae cancellous bone of flunride-treated rats
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摘要:目的 研究氟对大鼠腰椎松质骨超微结构的影响。方法 32只体重为(80±10)g的健康雌性Wistar大鼠分别经饮水给不同剂量(0, 25, 50, 100mgF-/L)的氟, 染毒3个月。处死动物后, 立即取第2腰椎, 剔除表面附着的软组织, 沿正中线作矢状剖开, 暴露骨髓腔, 用生理盐水冲洗后, 经固定、脱水、脱脂、干燥、喷金等处理步骤, 用扫描电镜观察松质骨超微结构改变。结果 随着染氟剂量的增加, 大鼠腰椎椎体松质骨骨量明显增加, 骨小梁数目增加, 表面凸凹不平、小梁不规则变厚、变粗, 骨小梁裂纹和微骨折较多见, 骨吸收陷窝缩小、变浅。胶原纤维出现板结硬化样改变和较宽的不规则裂隙。结论 长期过量摄入氟可使大鼠腰椎椎体松质骨的骨形成增加, 但所形成的骨排列多不规则, 骨小梁裂纹和微骨折较多见, 导致骨的质和量分离现象。Abstract:Objective To investigate effect of fluoride on the ultrastructure of rat lumbar vertebrae cancellous bone.Methods Sodium fluoride was administered to female Wistar rats through drinking water with three doses of 25, 50 and 100 mg F-/L for a period of 3 months. Cancellous bone from the second lumbar vertebrae was studied morphologically with a scanning electron microscope.Results The significant structural changes in bone trabecula and collegen fiber were observed in the fluoride-treated animals as compared with normal bone. With the increase of fluoride dose being increased, bone trabecula numbers were increased, the wall of bone trabecula was thickening, and the surface of bone trabecula was rough and uneven. The collegen fibers showed sclerosis-looking changes. Some wide crevices in bone trabecula could be observed under the scanning electron microscope.Conclusion Long-time exposure to high fluoride may cause more bone formation in rat lumbar vertebrae cancellous bone, but the new-formed bones were not well arranged. There were bone trabecula cracks and tiny fracture phenomena of separated quality and volume of bone trabecula in rat lumbar vertebrae cancellous bone could be seen.
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