依布哂啉纯度、杂质检测及安全性实验

Detection of purity and impurity of ebselen and security test

摘要:
目的检测合成的依布哂啉(EbselenEbs)的纯度、杂质并进行急性毒性实验。
方法 Ebs测定采用自建立的高效液相色谱法(HPLC), 杂质(苯胺)采用比色法, 急性毒性试验采用成年大鼠1次性灌胃, 观察24h内死亡率。
结果 Ebs纯度为99.95%~99.99%, 苯胺含量为0.00017%~0.00019%, 大鼠1次经口的LD₅₀为2355.05mg/kg。
结论 Ebs质量可靠, 安全性高, 可供开发研究。

Abstract:
Objective To understand the purity and the impurity of the synthesized ebselen (Ebs) and the acute toxicity.
Methods The Ebs was tested with HPLC, the impurity was detected with chromatometry and the acute test was done by adultratlav aged one time and the 24-hour mortality rate was observed.
Results The purity of Ebs was 99.95%-99.99%, the amount of phenylamine was 0.000 17%-0.000 19%, the LD₅₀ of the rat lavaged by mouth one time was 2 355.05mg/kg.
Conclusion The quality of Ebs was reliable, the security was high, and it could be used for exploitation and study.