香烟烟雾溶液对人淋巴细胞的损伤及修复

DNA damage and repair of human lymphocytes treated with cigarette smoke solution

摘要:
目的了解香烟烟雾溶液作用于人淋巴细胞后DNA的损伤及修复状况.
方法以8×10^-3支/ml香烟烟雾溶液作用于正常人淋巴细胞, 用彗星试验检测0, 0.5, 1, 2, 3, 4, 5, 6h时点细胞的DNA损伤状况.
结果与0h组相比, 2h至6h组彗星尾长均显著增长(P < 0.001), 3h达到最长, 之后逐渐降低, 但到6h未恢复至正常水平.
结论香烟烟雾溶液能直接引起人淋巴细胞的DNA损伤, 且细胞能在一定程度上修复这种损伤.

Abstract:
Objective To learn DNA damage and its repair in normal human lymphocytes treated with cigarette smoke solution(CSS).
Methods Human lymphocytes were exposed to 8×10^-3 cig/ml CSS for0, 0.5, 1, 2, 3, 4, 5and6 hrespectively, then DNA damage was evaluated by comet assay.
Results T ail leng ths of comet in groups exposed to CSS from 2 to 6 h were significantly longer than that of the control(0 h)(P < 0.01).It reached the longest at 3 h and decreased subse-quently.However, it did not reduce to normal level at 6 h.
Conclusion CSS could induce DNA damage of human lymphocytes, and the damage could be repaired to some extent.