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张晓智, 陈葳, 李旭, 徐海伟. BRL-CM小鼠胚胎干细胞及体外诱导的研究[J]. 中国公共卫生, 2003, 19(11): 1333-1334.
引用本文: 张晓智, 陈葳, 李旭, 徐海伟. BRL-CM小鼠胚胎干细胞及体外诱导的研究[J]. 中国公共卫生, 2003, 19(11): 1333-1334.
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ZHANG Xiao-zhi, CHEN Wei, LI Xu, . Study on differention of mouse embryonic stem cells cultured in BRL-CM to neural precursor cells in vitro[J]. Chinese Journal of Public Health, 2003, 19(11): 1333-1334.
Citation: ZHANG Xiao-zhi, CHEN Wei, LI Xu, . Study on differention of mouse embryonic stem cells cultured in BRL-CM to neural precursor cells in vitro[J]. Chinese Journal of Public Health, 2003, 19(11): 1333-1334.
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BRL-CM小鼠胚胎干细胞及体外诱导的研究

Study on differention of mouse embryonic stem cells cultured in BRL-CM to neural precursor cells in vitro

    摘要
  • 摘要:
      目的   探讨以Buffalo大鼠肝细胞培养上清(BRL-CM)替代含LIF培养液体外培养小鼠胚胎干细胞(ESC)的可行性; 无血清体外诱导ESC为神经前体细胞(NPC).
      方法   以BRL-CM维持ESC生长并抑制其分化, 用含LIF的培养液作为对照, 硝基四氮唑蓝/5-溴-4氯-3-吲哚基磷酸(NBT/BCIP)显色检测碱性磷酸酶.采用N2选择性无血清培养法获取NPC, 免疫组化法检测巢蛋白(Nestin)鉴定.
      结果   BRL-CM与含LIP的培养液一样能维持ESC未分化状态, 无血清培养基诱导ESC后获得细胞的86%为NPC.
      结论   BRL-CM可替代LIF用于ESC培养, 且经济、简便.采用N2选择性培养基可获得较高纯度的NPC.

     

    Abstract:
      Objective   To investigate whether buffalo ratliver cell-conditioned medium(BRL-CM)can be used as the cultur condition of embryonic stem cells(ES cells), and to get more pure neural precursor cells(NPC)of treatment.
      Methods   Mouse embryonic stem cells were cultured in BRL-CM and containing LIF medium respectively, NPC were selectively cultured in serum-free medium.Alkaline phosphatase activity was visualized with NBT/BCIP and nest in antigen was detected with immunocy tochemical methods.
      Results   BRL-CM could be used as an efficiency culture condition insted of LIF in ES cells culture.About 86% NPC were obtained from ES cells in the serum-free culture condition.
      Conclusion   BRL-CM can replace LIF and with more simple and economy; high purity NPC could be induced from ES cells with serum-free culture method.

     

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