高级检索
卢次勇, 凌文华, 马静, 唐志红, 吴聪娥. 低密度脂蛋白影响巨噬细胞的SAPK信号通路[J]. 中国公共卫生, 2003, 19(10): 1171-1173.
引用本文: 卢次勇, 凌文华, 马静, 唐志红, 吴聪娥. 低密度脂蛋白影响巨噬细胞的SAPK信号通路[J]. 中国公共卫生, 2003, 19(10): 1171-1173.
shu
LU Ci-yong, LING Wen-hua, MA Jing, . Study on signal pathway on function changing of macrophages induced by oxidized low density lipoprotein[J]. Chinese Journal of Public Health, 2003, 19(10): 1171-1173.
Citation: LU Ci-yong, LING Wen-hua, MA Jing, . Study on signal pathway on function changing of macrophages induced by oxidized low density lipoprotein[J]. Chinese Journal of Public Health, 2003, 19(10): 1171-1173.
shu

低密度脂蛋白影响巨噬细胞的SAPK信号通路

Study on signal pathway on function changing of macrophages induced by oxidized low density lipoprotein

    摘要
  • 摘要:
      目的   研究氧化型低密度脂蛋白(oxidized lowdensity lipoprotein, Ox-LDL)对巨噬细胞增殖、坏死及凋亡影响的非清道夫受体途径, 及对应激活化蛋白激酶(stress-activated protein kinase, SAPK)信号通路的影响。
      方法  采用3个时间水平(24, 48, 72h)、4个剂量水平(0, 50, 100, 200mg/L)的两因素析因设计, 观察Ox-LDL对清道夫受体A(scavenger receptorA, SRA)基因敲除小鼠巨噬细胞增殖、坏死及凋亡影响的时间、剂量效应关系; Ox-LDL对该细胞SAPK活性的影响。
      结果   Ox-LDL可诱导SRA基因敲除小鼠巨噬细胞增殖、凋亡及坏死, 并与其作用浓度和剂量相关(P < 0.01), 同时Ox-LDL可引起SAPK活性水平发生变化。
      结论   Ox-LDL对SRA基因敲除小鼠腹腔巨噬细胞的影响主要为诱导增殖作用, 高浓度时伴有一定致凋亡、坏死作用, 其致细胞增殖、凋亡作用可能部分与SAPK信号途径有关, 提示非清道夫受体途径在Ox-LDL导致巨噬细胞功能改变过程中可能起重要作用。

     

    Abstract:
      Objective   To study the effects of oxidized low density lipoprotein on proliferation, apoptosis and necrosis of macrophages via the possible mechanism of signal transduction unrelated to scavenger receptor pathway.The role of SAPK (Stress-activated protein kinase) signal pathway were studied.
      Methods   Two-factorial experiment was designed to explore the influence of four dosage levels of Ox-LDL (0, 50, 100, 200 mg/L) at three different stages of exposure (24, 48 and 72 hr) on proliferation apoptosis and necrosis of macro phage cells of SRA (scavenger receptor A) knockout mice. Activity of SAP K in the macrophage cells of SRA knockout mice was observed with different exposure level of Ox-LDL at different times.
      Results   The apoptosis and necrosis of macrophages of SRA knockout mice induced by Ox-LDL was associated with both the exposure level and time (P < 0.01); Ox-LDL could induced the proliferation of the macrophages of SRA knockout mice, which was significantly associated with the dosage and time (P < 0.01). The activities of SAPK were changed at different dose of Ox-LDL.
      Conclusion   Proliferation was the main response to Ox-LDL, and apoptosis and necrosis were also observed for higher exposure of Ox-LDL in macrophages of SRA knockout mice. This proliferation and apoptosis induced by Ox-L DL might be partly mediated by SAPK signal pathways, which implicated that other ways might play the role in the Ox-LDL induced proliferation and apoptosis of macrophage with scavenger receptor deficiency.

     

    • HTML全文
    • 参考文献(9)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回