Abstract:
Objective To recognize and clone the novel genes of Schistosoma japonicum (Sj).
Methods The ESTs obtained in our laboratory were analyzed by homologous searching, the novel genes were recognized. Accor ding to the interested EST, the full-length cDNA of the novel gene was obtained by 3' RACE PCR. The cDNA sequence were cloned into pGEX-4T-1 vector and analyzed by bioinformatics.
Results A new cDNA sequence was obtained, the sequence contains a fulllength sequence with 654bp ORF, which encoded 217 amino acid residues. The new gene was identified to be the gene encoding casein kinase Ⅱ beta subunit. The sequences were cloned into pGEX-4T-1 vector and identified by restriction analysis and sequencing.
Conclusion The full-length cDNA sequences encoding Sj casein kinase Ⅱ beta subunit were firstly sequenced and cloned, which give the basis for further study.
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