山东省HIV-1C亚型毒株的分子流行病学研究

Molecular epidemiologic analysis on HIV-1 subtype Cstrains in Shandong province

摘要:
目的了解HIV-1C亚型毒株在山东省不同人群中的流行传播情况, 分析其传染来源和传播规律。
方法采集7份HIV-1感染者的外周静脉防凝血, 提取前病毒DNA进行体外扩增, 获得包膜蛋白(env)基因的核酸片段, 并对其C2-V3及邻区的核苷酸进行测定和分析。
结果 7份血样均为HIV-1C亚型感染, 系统树和基因距离分析发现, 其中5株靠近07-BC重组毒株, 2株靠近08-BC重组毒株。
结论山东省C亚型有重组株存在的可能, 且人群分布广, 传播途径多样, 具有广泛传播的潜在危险。

Abstract:
Objective To investigate the prevalent status of HIV-1 subtype C epidemic among different populations in Shandong province.
Methods DNA fragments of HIV-1 env gene were amplified by nested PCR from persons infected with HIV-1.C2-V3 and adjacent region were sequenced.
Results These 7 cases were confirmed to be infected with HIV-1 subetype C strain, 5 strains are nearer to 07-BC, and 2 strains are nearer to 08-BC in phylogenetic tree.
Conclusion There may be CRFs of HIV-1 C strain in Shandong, C subtype distributed in 4 kinds of populations and had 3 various routes to transmit.