氟化钠对体外培养大鼠肾细胞增殖的影响

Effect sodium fluoride on proliferation of nephrocyte in rats in vitro

摘要:
目的研究不同浓度NaF对体外培养大鼠肾细胞增殖反应的影响以及V_E、Vc、Se和GSH对其保护效应。
方法大鼠肾细胞分离培养, 分别施加不同浓度NaF、一定浓度V_E、Vc、Se和GSH, 采用ELISA方法测定OD值, 计算肾细胞增殖率。
结果 NaF对肾细胞增殖反应具有双向性作用, 即低浓度刺激、高浓度抑制。
结论一定浓度V_E、Vc和GSH能拮抗1mmol/LNaF对大鼠肾细胞增殖反应的抑制作用。

Abstract:
Objective To study the effect of NaF on proliferation of nephrocyte and protective efficiency of V_E, Vc, Se and GSH.
Methods Adding different concentrations of NaF, V_E(25μmol/L), Vc(50μmol/L), Se(5μmol/L)and GSH (1mmol/L)into cultured nephrocyte using ELISA to determine the level of OD, and computing the proliferation rate of nephrocyte.
Results NaF has two-side action on the proliferation of nephrocyte.
Conclusion V_E, Vc and GSH can antagonize the toxic effect of NaF(1 mmol/L)on nephrocyte.