缺氧对梭曼中毒大鼠心脏胆碱能系统的影响

刘辉; 刘勇; 王仕丽; 吴强; 郭魁亮

缺氧对梭曼中毒大鼠心脏胆碱能系统的影响

Effects of soman intoxication under high altitude hypoxia on heart cholinergic system of rat

摘要

摘要:
目的探讨高原缺氧条件下梭曼中毒对大鼠心肌乙酰胆碱酯酶(AchE)活性和m₂受体表达的影响。
方法实验大鼠随机分为正常对照组、高原缺氧组、平原中毒组和缺氧中毒组。利用DTNB法和RT-PCR检测大鼠心肌AchE活性和m₂受体mRNA的表达。
结果模拟高原缺氧大鼠心肌AchE活性出现不同程度的升高, 心肌m₂受体mRNA表达增强; 缺氧条件下梭曼中毒时大鼠心肌AchE活性降低, m₂受体表达减弱, 但与平原中毒组比较, 缺氧中毒组大鼠AchE活性和m₂受体mRNA的表达有所上升(P < 0.05)。
结论高原缺氧和梭曼中毒均明显干扰大鼠心脏胆碱能神经系统, 高原缺氧条件下胆碱能系统功能上调, 可能是高原地区有机磷毒剂毒性升高的重要原因之一。

Abstract:
Objective To study the effects of soman intoxication under high altitude hypoxia on the activity of AchE and the expression of m₂ receptor mRNA.
Methods Experimental Wistar rats were randomly divided into four groups: normal control group, high altitude hypoxia group, plain soman intoxication group and soman intoxication combined hypoxia group.The activity of AchE and the expression of m₂ receptor mRNA in rats myocardium were measured with DTNB method and RT-PCR.
Results Hypoxia increased the activity of AchE in different degrees, and the expression of m₂ receptor also increased; during soman intoxication under high altitude hypoxia, the expression of m₂ receptor mRNA enhanced remarkably compared with plain soman intoxication group.
Conclusion Both high altitude hypoxia group and soman intoxication significantly interfered with the functions of the cholinergic nervous system in the heart.The up-regulation of cholinergic system may play an important role in the toxicity increasing of organophosphate compounds at high altitude.

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