HSV-2DNA疫苗诱导小鼠免疫应答研究

Immune response induced by herpes simplex virus-2 DNA vaccine in mice

摘要:
目的探讨含有单纯疱疹病毒Ⅱ型(HSV-2)糖蛋白D全基因序列的重组质粒作为DNA疫苗的可能性。
方法采用PCR方法获得HSV-2gD片段, 构建含HSV-gD片段的重组质粒pcDNA3-gD。重组质粒作为DNA疫苗免疫小鼠, 观察其对小鼠免疫效果及保护作用。
结果重组质粒pcCDNA3-gD免疫小鼠后可产生特异性抗体, 可保护小鼠免受HSV-2的致死性攻击, 存活率达75%。
结论重组质粒pcDNA3-gD可诱导小鼠产生特异性免疫反应, 可以作为HSV的候选DNA疫苗。

Abstract:
Objective To evaluate the protective efficacy of DNA vaccine expressing herpes simplex type 2(HSV-2) full-length glycoprotein D(gD).
Methods The whole encoding sequence of gD was amplified from HSV-2 genome by PCR, and then cloned into downstream of PCMV of pcDNA3, and resulted in the HSV-2 DNA vaccince-pcDNA3-gD.pcDNA3-gD was inoculated to mice by bilateral intramuscular injection into the rear legs.
Results The mice inoculated with pcDNA3-gD could produce specific antibodies and survive after the lethal challenge with HSV-2.The rate of survival rate was 75%.
Conclusion pcDNA3-gD can induce specific immune response and may be a candidate DNA vaccine D.