低剂量铅对原代培养的海马神经元的影响

Impact of low-level lead on growth and survival of primary cultured hippocampus neurons

摘要:
目的研究低剂量铅对原代培养的海马神经元生长及存活的影响.
方法建立新生大鼠海马神经元原代无血清培养技术, 通过不同浓度PbCl₂染毒后, 于不同时间点观察神经细胞的生长和存活情况.
结果极低浓度的铅(10^-7mol/L)即可引起神经元出芽延迟, 突起长度缩短, 细胞存活率下降, 随剂量增加和时间延长, 毒效应越严重.
结论低剂量铅具有抑制神经元生长和存活的毒性.

Abstract:
Objective To study the impact of low-level lead on the growth and survival of primary cultured hippocampus neurons.
Methods To establish the technique of primary culturing hippocampus neurons of newborn rat with serum-free medium.Being treated with different dosage of PbCl₂, the growth and survival of the cultured cells were observed at different time-point.
Results Even low level of 10^-7 mol/L Pb²⁺ could delay the neurite's outgrowth, shorten the neurite's length and decrease the neuron's survival rate.When Pb²⁺ increased, its toxic effects increased.
Conclusion Low-level lead inhibited the neurons'growth and survival.