9-羧酸蒽对人髓性白血病细胞株HL-60凋亡的研究

杜可军; 陈景元; 周士胜; 常文辉; 叶琳; 陈耀明

9-羧酸蒽对人髓性白血病细胞株HL-60凋亡的研究

Study on apoptosis induced by anthracene-9-carboxylic acid in HL-60 cells

摘要

摘要:
目的研究氯通道抑制剂9-羧酸蒽(9-AC)对人髓性白血病细胞株HL-60的影响, 并对相关机制进行探讨.
方法体外培养人白血病细胞株HL-60, 采用透射电子显微镜、DNA琼脂糖凝胶电泳、流式细胞仪等观察9-A对HL-60细胞的作用.
结果在5×10^-4mol/L9-AC作用48h后, 细胞发生明显的改变.电镜下细胞形成典型的凋亡小体.DNA琼脂糖凝胶电泳出现特征性梯形条带.流式细胞仪分析表明, 实验组HL-60细胞出现明显的亚二倍体峰-凋亡峰.
结论 9-AC具有明显的诱导白血病细胞HL-60凋亡的作用.

Abstract:
Objective To study the effects of 9-A Con apoptosis in HL-60 cells and possible intracellular factors involved in the process.
Methods HL-60 cells were treated with 9-AC to induce cell apotosis.Three international accepted criteria for monitoring apoptosis were adopted, including transmission electron microscopy, agarosegel DNA electrophoresis, and fluorescence-activated cell sorter(FACS).
Results 9-AC could induce apoptosis of HL-60 cells, with its concentration most opt imal at 5×10^-4 mol/L.Forty-eight hours treatment of HL-60 cells with 9-AC at above concentration induced remar kable apoptotic phenomena as typified as: Ultrastr uctural changes formed the so-called apoptotic bodies, typical DNA ladder, symbolizing inter nucleosomal fragmentation of DNA were observed by using agarosegel electrophoresis of isolated DNA from HL-60 cells.FACS analysis revealed markedly apoptotic peak-subdiploid peak.
Conclusion 9-AC could induce apoptosis of HL-60 cells.

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