Abstract:
Objective To investigate the influence of genistein on proliferation, cell cycle, and apoptosis of breast cancer cell MDA-MB-435S.
Methods Time and dose effects of genistein were detected using M TT assay.The influence of different time and dose on the cell cycle was observed by flow cytometry.The relationship of dose effects of apoptosis was observed in fluorescent microscope with acridium orange/ethidium bromide staining.
Results Genistein inhibited the proliferation of MDA-MB-435S cell line, the higher the dose and/or the longer the time, the more obvious the effects.Furthermore, genistein could block cell cycle progression in G2-M, which varied with different dose and time.Genistein induced cell apoptosis, which depended on the dose.
Conclusion Genstein could inhibite the proliferation of human breast cancer's MDA -MB-435S cell line in vitro, the role of genistein may be the two pathways of apoptosis and cell cycle blocking.