镁对过氧化氢诱导内皮细胞热休克基因表达的影响

吕晓华; 王瑞淑

镁对过氧化氢诱导内皮细胞热休克基因表达的影响

Effect of magnesium supplementation on heat shock gene expression in endothelial cells induced by H2O2

WANG Rui-shu,

摘要

摘要:
目的观察Mg²⁺对H₂O₂诱导的内皮细胞热休克基因表达的影响.
方法在改进人脐静脉内皮细胞培养方法的基础上, 分为: (1)正常对照组; (2)H₂O₂组; (3)补镁组; (4)H₂O₂+Mg²⁺组.TBA法检测细胞脂质过氧化水平, 免疫组织化学法检测细胞热休克蛋白70(HSP70)的表达水平.
结果与H₂O₂组相比, H₂O₂+Mg²⁺各剂量组硫代巴比妥酸反应物质(TBARS)显著降低, P < 0.05;H₂O₂+Mg²⁺各剂量组HSP70基因表达强于H₂O₂组(P < 005, P < 001), 且随Mg²⁺浓度升高, HSP70基因表达逐渐增强.
结论 Mg²⁺促进热休克基因的表达可能与其在氧化应激反应中对细胞的保护作用有关.

Abstract:
Objective The effect of magnesium on heat shock gene expression in endothelial cells induced by H₂O₂ was studied.
Methods The cultured human umbilical veinendothelial cells were divided into 4 groups(group of control, group of H₂O₂, group of Mg²⁺ and group of H₂O₂+Mg²⁺).The extent of cellular lipid peroxides was deter mined by measuring the for mation of TBARS, and expression of HSP70 by the method of immunohisto chemistry staining.
Results Compared with the group of H₂O₂, the formation of TBARS of groups H₂O₂+Mg²⁺ at all doses was decreased(P < 0.05), and the expression of HSP in the groups H₂O₂+Mg²⁺ at all doses increased significantly(P < 0.05, P < 0.01).
Conclusion The effect of magnesium on protecting cells from oxidative stress was probably related to its action on heat shock gene expression.

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