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张宏伟, 徐永俊, 张丽霞, 丁鹏, 陈嵘. 小鼠ScFv基因片段的构建[J]. 中国公共卫生, 2003, 19(5): 567-568.
引用本文: 张宏伟, 徐永俊, 张丽霞, 丁鹏, 陈嵘. 小鼠ScFv基因片段的构建[J]. 中国公共卫生, 2003, 19(5): 567-568.
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ZHANG Hong-wei, XU Yong-jun, ZHANG Li-xia, . Construction of ScFv gene fragment of mouse[J]. Chinese Journal of Public Health, 2003, 19(5): 567-568.
Citation: ZHANG Hong-wei, XU Yong-jun, ZHANG Li-xia, . Construction of ScFv gene fragment of mouse[J]. Chinese Journal of Public Health, 2003, 19(5): 567-568.
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小鼠ScFv基因片段的构建

Construction of ScFv gene fragment of mouse

    摘要
  • 摘要:
      目的   利用分子重组技术构建小鼠单链抗体片段(ScFv).
      方法   采用PCR反应从小鼠脾细胞扩增鼠免疫球蛋白的重链和轻链可变区, 利用连结引物将重链和轻链DNA组装成单链抗体(ScFv)片段.
      结果   克隆出VH, VL基因, 用Linker将VH, VL连接起来后的ScFv构建成功, 其分子量为750bp.
      结论   ScFv分子小, 异源性低, 可以结合与噬菌体载体中形成融合蛋白表达, 该技术是抗体制备的一种简便、可靠的方法.

     

    Abstract:
      Objective   To assemble the antibody heavy chain and light chain variable domains by the molecular recombinant technology.
      Methods   Using the PCR, immuno globulin variable(V)region genes are amplified from spleen cells.The VH(variable heav y)and VL(variable light)genes are then linked into ScFv.
      Results   The VH and VL gene are cloned and the ScFv gene fragment are assembled.The fregment is 750 bp.
      Conclusion   ScFv is small molecular and ligated to phage vector and screen fusion protein.The technology is a simple and potential method to make antibody.

     

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