野生山葡多酚对小鼠组织的抗氧化作用研究

Study on anti-oxidative action on tissues of mice

摘要:
目的研究野生山葡萄多酚类物质(PVA)对小鼠组织的抗氧化作用.
方法采用TBA法、NBT法和荧光法, 分别测定小鼠心、肝、肾、脑组织中MDA含量、SOD活性及脂褐质含量.
结果 PVA(200、400mg/kg·d×30d)显著降低, 以上各组织MDA及脂褐质的含量(P < 0.05), PVA(400mg/kg·d×30d)极显著提高心、脑组织SOD活性和降低心、脑组织脂褐质及MDA含量(P < 0.01).
结论 PVA能有效地提高机体组织抗氧化能力, 防止组织脂质过氧化损伤.

Abstract:
Objective To study the anti-oxidative action of PVA on the tissues of mice.
Methods Contents of malon-dialdehyde(MDA), superoxide dismutase(SOD)and lipofuscin in the heart, liver, brain and kidney of mice were measured using TBA, NBT and fluor escent technique respectively.
Results PVA(200, 400 mg/kg·d 30 d)could decrease significantly the content of MDA and the content of lipofuscin in all tissues(P < 0.05).PV A(400 mg/kg·d 30 d)could increase significantly the activity of SOD and decrease significantly the content of lipofuscin and MDA in heart, brain of mice.
Conclusion PVA could increase the anti-oxidative action in vivo significantly and prevent lipid peroxidation damages in tissues.