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余日安, 陈学敏, 吴赤蓬, 范存欣, 鲁文清. 镉对大鼠肝细胞增殖周期和DNA合成的影响[J]. 中国公共卫生, 2003, 19(3): 298-300.
引用本文: 余日安, 陈学敏, 吴赤蓬, 范存欣, 鲁文清. 镉对大鼠肝细胞增殖周期和DNA合成的影响[J]. 中国公共卫生, 2003, 19(3): 298-300.
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YU Ri-an, CHEN Xue-min, WU Chi-peng, . Effects of cadmium on rat hepatocellular DNA synthesis and cell cycle changes[J]. Chinese Journal of Public Health, 2003, 19(3): 298-300.
Citation: YU Ri-an, CHEN Xue-min, WU Chi-peng, . Effects of cadmium on rat hepatocellular DNA synthesis and cell cycle changes[J]. Chinese Journal of Public Health, 2003, 19(3): 298-300.
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镉对大鼠肝细胞增殖周期和DNA合成的影响

Effects of cadmium on rat hepatocellular DNA synthesis and cell cycle changes

    摘要
  • 摘要:
      目的   研究氯化镉对大鼠肝细胞增殖周期和DNA合成的影响。
      方法   选用雄性SD大鼠, 每组5只, 用5, 10和20μmol/kg的氯化镉腹腔注射染毒。用流式细胞术研究大鼠肝细胞增殖周期和DNA相对含量(DNARC), 用单细胞凝胶电泳检测DNA损伤。
      结果   5, 10和20μmol/kg的氯化镉均可使大鼠肝细胞G0/G1期细胞显着减少, S期和G2/M期细胞以及增殖指数变化不明显。5, 10和20μmol/kg的氯化镉可引起大鼠肝细胞DNARC显着下降, DNA损伤率较对照组显着增高, 而且DNARC和DNA损伤率之间存在负相关关系, 随DNA损伤率的增高, DNARC下降, 相关系数为: -0.9990(P < 0.01)。
      结论   一定剂量的氯化镉不仅改变了大鼠肝细胞的增殖周期, 还引起DNA损伤并影响DNA合成使DNA相对含量显著下降。

     

    Abstract:
      Objective   To explore effects of cadmium on rathepatocellular DNA synthesis and cell cycle changes.
      Methods   Cadmium chloride at the dose of 5, 10 and 20 μmol/kg were given to rats by i.p.and there were 5 male SD rats in each group.Hepato cellular DNA damage was measured with single cell gel electrophoresis(or comet assay), DNArelative content (DNARC)and cell cycle were detected by flow cytometry.
      Results   At the dose of 5, 10 and 20 μmol/kg, cadmium chloride could reduce cell number of G0/G1 period significantly, the changes of proliferation index and cell number in Sand G2/M period were not obvious.Cadmium chloride at the dose of 5, 10 and 20 μmol/kg could increase DNA damage rate and decrease DNARCsignificantly.There were negative correlation between DNA damage rate and DNA relative content.Corelative coefficient was -0.999 0(P < 0.01).
      Conclusion   Cadmium at certain dose could change cell cycle and interfere with DNAsy nthesis through increasing DNA damage rate and decreasing DNA relative content in rat's liver cells.

     

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