广东省HIV-1流行株的基因亚型分析
Genetic subtyping of human immunodeficiency virus-1 in Guangdong province
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摘要:目的 了解广东省人免疫缺陷病毒-1(HIV-1)感染毒株的亚型分布情况。方法 用巢式聚合酶链反应(nPCR)技术, 对1998年初至2002年2月间广东省内13例HIV-1感染者血标本进行膜蛋白基因(env)部分片段扩增, 将扩增产物克隆至T载体上并进行序列分析。结果 在11例扩增成功的血标本中, B亚型6例, 其中3例来自献血人员, 1例为性乱人员和2例为吸毒人员; E亚型5例, 其中3例为性乱人员, 1例来自吸毒人员, 1例来自献血人员。5例E亚型基因离散率为(6.6±3.8)%; 6例B亚型基因基因离散率为(7.6±1.9)%, 与来自泰国和国内的B亚型代表株最接近。结论 广东省至少有HIV-1B和E亚型毒株流行, 且分布广泛, 来源复杂。Abstract:Objective To reveal the distribution of subtypes of HIV-1 in Guangdong Province.Methods The env gene of HIV was amplified by nested polymerase chain reaction(nPCR)in 11 HIV-1 infected individuals from 1998 to Feb 2002.The PCR products were cloned to T-vector and were sequenced automatically.Results Among eleven HIV positive blood samples, 6 were subtype B, including 3 blood donors, 1 sex workers and 2 IDUs.Five cases were subtype E, including 3 sex workers, 1 blood donors and 1 IDUs.The genetic distance of subtype B and E was(7.6±1.9)% and(6.6±3.8)%, respectively.The 6 subtype B strains were closed to the type B in Thailand or China.Conclusion There were at least subtype B and E of HIV-1 strains in Guangdong Province and they were from different sources.
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