应用非放射性探针检测HCMV感染的研究

A Study on Detecting HCMV with D goxigenin-UTP Labeled Probe

摘要: 应用地高辛配基标记HCMVDNAJ片断为探针，采用斑点杂交试验检测唾液及尿液中HCMVDNA.此方法的最低检测阈值为0.5pg,与HSV-1、HSV-2、RuV、DNA无交叉反应。检测17例患儿的唾液和尿液HCMVDNA,其阳性率分别为50%和17.6%,唾液中HCMVDNA阳性率显着高于尿液；检测136对正常母婴配对唾液标本，HCMVDNA阳性率分别为46.3%和9.6%,其相应血清用ELISA检测HCMV-IgM抗体，阳性率分别为2.2%和0%.此结果提示，同时检测唾液中病毒DNA及血清中IgM抗体，对临床及流行病学筛检试验判断是否有激活感染及感染的早、晚期有重要意义。

Abstract: Wedetected HCMV DNA withdigoxigoxigen in UTP labeled probe of HCMV DNA nentbydot to thyb ridization in saliva and urine The bwest threshold of detecting HCMV DNA was 0.5pg,no cross reactin with HSV^-1 HSV^-2 Ru VDNA was found HCMV DNA infection rates were and17.6% in 17 sick children saliva and urine and the infection rate in saliva was signfic.