军团菌16SrRNA聚合酶链反应方法的建立及应用

Establishment and Application of a 16SrRNA Polymerase Chain Reaction Assay for Detection of Legionella Species

摘要: 建立了16SrRNA基因检测军团菌的多聚酶链反应方法。扩增参考菌株染色体DNA（L.Pneumophila1～14、L.bozemanni、L.dumofi、L.longbeachae、L.micdadei、L.jordanis），均可检出出386bp的基因片段，敏感性为103cfu/ml（平均值）;而扩增9株非军团菌均为阴性。对模拟血标本的检测，其敏感性可达102cfu/ml.应用本检测系统检测了24例临床标本，包括19份血、5份胸水，阳性率为70.8%（17/24）。与血清学检测和临床诊断治疗结果相符合。上述结果表明该方法敏感、特异、快速、简便。

Abstract: A 16SrRNA Polymerase Chain reaction(PCR) assay for the detection of Legionella Species was established. A 386bp fragment of the 16SrRNA gene could be detected by amplifing genomic DNA of reference strains(L.Pneumophila1～14、L.bozemanni、L.dumofi、L.longbeachae、L.micdadei、L.jordanis). 9 non-legionella strains couldn't produced a positive am-plified feagment. The sensitive for reference strains and blood were 103 cfu/ml(average) and 102 cfu/ml respectively. This PCRsy stem has been successful in detecting 24 clinical samples, the positive rate was 70.8%(17/24). A bove mentioned result showed that this assay was sensitive, rapid, spesific and easy toper form.