Abstract: To investigate the prevelence of anti-HGV antibody among different populations and patients with various liver diseases.Methods Anti-HGV,HBsAg,anti-HBs,anti-HBc and anti-HCV in sera were detected by enzyme-linked immunoassays(EIA).Anti-HGV positive sera were further tested for HGV RNA by a reverse transcription nested polymerase chain reaction(RT-nPCR).The prevalence of anti-HGV antibody in different populations and patients with various liver diseases were as folow s:4/395(1.0%)in general population,10/278(3.6%)in blood donors,70/541(12.9%)in plasmas donor,22/162(13.6%)inint ravenous drug addicts,12/56(21.4%)in hemodialysis patients,3/25(12.0%)in HBsAg carriers,11/40(26.2%)in chronic hepatitis B,17/136(12.5%)in chronic hepatitis C,4/11(36.4%)in cirrhosis and 3/16(18.8%)in chronic non-A to E hepatit is patients.Among 97 anti-HG V positive individuals,64(66%)were HGV RNA positive,indicating the possibility of use of anti-HGV EIA for the screening of blood donors to reduce post-transfusion HGV infection.