Development of neuron glucose oxygen deprivation model in evaluation on anoxia endurance function of health food

Objective To develop a mouse cortical neuron glucose oxygen deprivation model to evalua teanoxia endurance(acute hypoxic ischemic damage)function of health food invitro.Methods Serum pharmacology method was adopted to prepare mouse serum containing taurine.Prmiary cortical neurons from neonate mouse(with in 24hr)were cultured and treated with different concentrations of the serumon the 8th day under the deprivation of oxygen and glucose.Then the contents of lactate dehydrogenase(LDH)and superoxide dismutase(SOD),and the viabitity of the neurons were determined and compared with those of the mice with acute brain hypoxic ischemic damage.Results The LDH levels for moderate and high dose taurine containing serumtreatment groups were 5.97±0.85 and 5 .68±1.55 U/g²prot and significantly lower than that of blank control(8.27±1.54 U/g grot,P<0.05).For low,moderate,and high dose taurine containing serumtreamtent groups,the SOD levels were 6.83±0.65,6.95±0.6,and 7.55±0.82 U/mg grot and the viability of the neurons were 90.66%,89.04%,and 91.92%,which both significantly higher than those of blank serum control(5.57±0.89 U/mg²prot,75.74%;P<0.05 for all).The survival time of the mice in low,mode rate,and high dose taurine complex treatment groups were 173s,19.5s,and 18.2 s and were significantly longer than that of in pure water treamtent group(P<0.05).Conclusion Taurine containing mouse serum has a protective effect on the cultured new born mouse cortical neurons.The results suggest that oxygen/glucose deprivation model of mouse cortical neuron can be used to evaluate the function of health food againstacute hypoxic-ischemic damage invitro.