Isolation, identification and partial purification of specific allergens from Aspergillus versicolor

Objective To isolate,identify and partially purify the allergens from fungal(Aspergillus versicolor).Methods Proteins from Aspergillus versicolor wereisolated and seperated by sodium dodecyl sulfate-polyacrylamide gelelectrophoresis(SDS-PAGE),and then identified by western-blot.The allergens of Aspergillus versicolor were consequently purified by ion exchange chromatography and gelchromatog raphy.Results More than 20 kinds of allergen prote in were separated from the crude extract of the fung us by SDS-PAG E and 6 allergens were identified by western blot.The allergens with molecular weight(MW)of 17kD,20kD and 62kD had higher binding rate.The allergens with molecular weight of 22 kD,35 kD a,76 kD a were minor allergens.The fraction containing the major allergen with MW of 20kD and the minor allergen with MW of 35kD were purified.Conclusion The studyiden tified major and mino rallergens of Aspergillus versicolor and partially purified the allergen ,swhich may provide a theoretic basis for allergy diagnosis and immuno the rapy.