Objective To establish and optimize a multiple reaction monitoring-mass spectrometry (MRM-MS) method for detection of intercellular adhesion molecule 1 (ICAM-1), which is a potential biomarker for ventricular septal defect.
Methods By means of UniProt database and the Skyline software we established a MRM-MS method to detect ICAM-1; then we screened the peptides and the ion pair of ICAM-1 and changed the collision energy value and declustering potential voltage to optimize the MRM-MS method.
Results Three peptides and 9 ion pairs were selected from 11 peptides and 88 ion pairs of ICAM-1 as the basis of quantitative determination. Detected with the optimized MRM-MS, the content of ICAM-1 protein in 10 serum samples was 318.40 ± 97.20 ng/ml, which was not significantly different from the result (330.30 ± 130.24 ng/ml) measured with enzyme-linked immunoassay (P > 0.05).
Conclusion By means of UniProt database and the Skyline software and several procedures, we established and optimized a MRM-MS method for the detection of ICAM-1.
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