Objective To explore dose- and time-effect of deltamethrin (DM) on alkaline phosphatase (AKP) activity in Blattella germanica and to provide references for the control of Blattella germanica.
Methods According to the national standard described in Efficacy Test and Evaluation Methods for Indoor Application of Hygienic Insecticide under Pesticide Registration (GB/T 13917.1 – 2009), totally 300 healthy post-emergence Blattella germanica of sensitive strain were randomly divided into 5 treatment groups exposed to different sublethal concentrations (1.54, 3.07, 6.14, 12.28, and 24.56 mg/L) of anhydrous acetone DM solution spray and a control group with only anhydrous acetone (50 in each group). The activities of AKP in Blattella germanica at 24, 48, 72, 96, 120, and 144 hours of the treatment were determined by using AKP Kit.
Results Dose-dependently and steadily inhibitive effect of DM on KAP were observed in Blattella germanica of all the 5 treatment groups with 72 hours. The maximum inhibitive rates were 23.9%, 29.5%, and 27.3% under the dosages of 3.07, 12.28, and 24.56 mg/L at 72-hour and the rates were 19.9% and 26.8% under the dosages of 1.54 and 6.14 mg/L at 96-hour after the DM treatment.
Conclusion The activity of AKP in Blattella germanica could be used as one of the indices for determining resistant level of Blattella germanica to pyrethroid insecticides because of the resistance of Blattella germanica to deltamethrin is due to the decrease in AKP sensitivity to the insecticide.
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