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Volume 38 Issue 1
Jan.  2022
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CHANG Xiao-hui, WAN Xiao-nan, ZHANG Jie, . Genotyping analysis on foodborn Listeria monocytogenes starins isolated in Beijing region[J]. Chinese Journal of Public Health, 2022, 38(1): 105-109. doi: 10.11847/zgggws1132726
Citation: CHANG Xiao-hui, WAN Xiao-nan, ZHANG Jie, . Genotyping analysis on foodborn Listeria monocytogenes starins isolated in Beijing region[J]. Chinese Journal of Public Health, 2022, 38(1): 105-109. doi: 10.11847/zgggws1132726

Genotyping analysis on foodborn Listeria monocytogenes starins isolated in Beijing region

doi: 10.11847/zgggws1132726
  • Received Date: 2020-10-12
    Available Online: 2021-08-12
  • Publish Date: 2022-01-10
  •   Objective  To examine genotype distribution of Listeria monocytogenes (Lm) strains isolated from meat product specimens in Beijing and genetic correlations among the strains with whole genome sequencing (WGS).   Methods  Totally 110 Lm strains were sequenced with WGS and analyzed with multilocus sequence typing (MLST). Gene assembly, prediction and function annotation for WGS results of the strains were performed using relevant softwares. The Comprehensive Antibiotic Research Database (CARD) and the Virulence Factors Database (VFDB) were used to screen drug resistance genes and pathogenic genes of the isolated Lm strains.   Results  The 110 Lm strains were classified into 17 sequence type (ST) types; among the 17 ST types, there was a unknown ST type (namely STnew1) and ST9 was the dominant ST type. The STnew1 has multiple drug resistance genes including genes against macrolides, aminoglycosides, oxazolidinones, chloramphenicols, tetracyclines, and trimethoprim and gene deletion of prfA, hpt, plcA, plcB, inlC, and hlyA. Of the 110 Lm strains, all carried the Listeria monocytogenes pathogenicity island (LIPI-1) gene, except for 6 non-pathogenic strains, and 20.9% carried drug resistance genes.   Conclusion  Multiple drug resistance genes are prevalence in Listeria monocytogenes strains isolated from meat products and LIPI-1 gene is dominant among the drug resistance genes. The results suggest that contamination of Listeria monocytogenes in meat products needs to be monitored and controlled to reduce the risk of foodborne diseases caused by the bacteria.
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  • [1]
    European Food Safety Authority, European Centre for Disease Prevention and Control. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015[J]. EFSA Journal, 2016, 14(12): e04634.
    [2]
    Hadjilouka A, Andritsos ND, Paramithiotis S, et al. Listeria monocytogenes serotype prevalence and biodiversity in diverse food products[J]. Journal of Food Protection, 2014, 77(12): 2115 – 2120.
    [3]
    Haase JK, Didelot X, Lecuit M, et al. The ubiquitous nature of Listeria monocytogenes clones: a large-scale multilocus sequence typing study[J]. Environmental Microbiology, 2014, 16(2): 405 – 416.
    [4]
    郭桂萍, 葛红梅, 王匀, 等. 单增李斯特菌检测技术研究进展[J]. 中国食物与营养, 2011, 17(3): 12 – 15. doi: 10.3969/j.issn.1006-9577.2011.03.003
    [5]
    Pontello M, Guaita A, Sala G, et al. Listeria monocytogenes serotypes in human infections (Italy, 2000 – 2010)[J]. Annali dell'Istituto Superiore di Sanità, 2012, 48(2): 146 – 150.
    [6]
    Kathariou S. Listeria monocytogenes virulence and pathogenicity, a food safety perspective[J]. Journal of Food Protection, 2002, 65(11): 1811 – 1829.
    [7]
    Vázquez-Boland JA, Kuhn M, Berche P, et al. Listeria pathogenesis and molecular virulence determinants[J]. Clinical Microbiology Reviews, 2001, 14(3): 584 – 640.
    [8]
    Scharff RL. Economic burden from health losses due to foodborne illness in the United States[J]. Journal of Food Protection, 2012, 75(1): 123 – 131.
    [9]
    Liu DY, Lawrence ML, Gorski L, et al. Listeria monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features[J]. Journal of Clinical Microbiology, 2006, 44(1): 214 – 217.
    [10]
    国家卫生和计划生育委员会, 国家食品药品监督管理总局. 4789.30 — 2016食品安全国家标准 食品微生物学检验 单核细胞增生李斯特氏菌检验[S]. 北京: 中国标准出版社, 2017.
    [11]
    Nyarko EB, Donnelly CW. Listeria monocytogenes: strain heterogeneity, methods, and challenges of subtyping[J]. Journal of Food Science, 2015, 80(12): M2868 – M2878.
    [12]
    Salcedo C, Arreaza L, Alcalá B, et al. Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clones[J]. Journal of Clinical Microbiology, 2003, 41(2): 757 – 762.
    [13]
    Wang Y, Zhao AL, Zhu RF, et al. Genetic diversity and molecular typing of Listeria monocytogenes in China[J]. BMC Microbiology, 2012, 12(1): 119.
    [14]
    Yin YL, Tan WJ, Wang GL, et al. Geographical and longitudinal analysis of Listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution[J]. Microbiological Research, 2015, 175: 84 – 92.
    [15]
    刘二龙, 袁慕云, 邓建英, 等. 出入境动物源性食品中单增李斯特菌的多位点序列分型分析[J]. 食品科学, 2016, 37(12): 212 – 216. doi: 10.7506/spkx1002-6630-201612038
    [16]
    霍哲, 王晨, 徐俊, 等. 2012 — 2015年北京市西城区单核细胞增生李斯特菌多位点序列分型及耐药研究[J]. 中国食品卫生杂志, 2017, 29(3): 289 – 293.
    [17]
    Knabel SJ, Reimer A, Verghese B, et al. Sequence typing confirms that a predominant Listeria monocytogenes clone caused human listeriosis cases and outbreaks in Canada from 1988 to 2010[J]. Journal of Clinical Microbiology, 2012, 50(5): 1748 – 1751.
    [18]
    Althaus D, Lehner A, Brisse S, et al. Characterization of Listeria monocytogenes strains isolated during 2011 – 2013 from human infections in Switzerland[J]. Foodborne Pathogens and Disease, 2014, 11(10): 753 – 758.
    [19]
    Morvan A, Moubareck C, Leclercq A, et al. Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France[J]. Antimicrobial Agents and Chemotherapy, 2010, 54(6): 2728 – 2731.
    [20]
    Bertsch D, Uruty A, Anderegg J, et al. Tn6198, a novel transposon containing the trimethoprim resistance gene dfrG embedded into a Tn916 element in Listeria monocytogenes[J]. Journal of Antimic-robial Chemotherapy, 2013, 68(5): 986 – 991.
    [21]
    闫韶飞, 裴晓燕, 杨大进, 等. 2012年中国食源性单核细胞增生李斯特菌耐药特征及多位点序列分型研究[J]. 中国食品卫生杂志, 2014, 26(6): 537 – 542.
    [22]
    马彦宁, 赵悦, 郭云昌, 等. 即食食品中单核细胞增生李斯特菌的血清学分型和毒力基因分析[J]. 中国食品卫生杂志, 2017, 29(1): 14 – 18.
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