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Volume 37 Issue 11
Nov.  2021
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BAI Pai-hui, LI Dan-ni, LIU Jun, . Role of γδT cells in lethal Plasmodium yoelii infection in BALB/c mice[J]. Chinese Journal of Public Health, 2021, 37(11): 1663-1666. doi: 10.11847/zgggws1132941
Citation: BAI Pai-hui, LI Dan-ni, LIU Jun, . Role of γδT cells in lethal Plasmodium yoelii infection in BALB/c mice[J]. Chinese Journal of Public Health, 2021, 37(11): 1663-1666. doi: 10.11847/zgggws1132941

Role of γδT cells in lethal Plasmodium yoelii infection in BALB/c mice

doi: 10.11847/zgggws1132941
  • Received Date: 2020-10-30
    Available Online: 2021-08-12
  • Publish Date: 2021-11-10
  •   Objective  To explore the role and mechanism of γδ T cells in immune response of BALB/c mice infected with the lethal Plasmodium yoelii 17XL (P.y 17XL).  Methods  Female BALB/c mice at 6 – 8 weeks of age were randomly divided into a normal control, P.y 17XL infection, and P.y 17XL infection and γδ T cell elimination group. The mice were intraperitoneally injected with P.y 17XL to infect 1×106 red blood cells per mouse. The mice of γδ T cell elimination group were intraperitoneally injected with anti-mouse TCR γ/δ monoclonal antibody (150 μg/mouse) for two consecutive days before P.y 17XL infection, while the mice of normal control and P.y 17XL infection group were injected with the same volume of isotype control antibody. The infection rate of red blood cells and survival rate of mice were monitored dynamically. The number of γδ T cells, dendritic cells (DCs), and type 1 helper (Th1) cells in spleen cell suspension were detected with flow cytometry at the third and fifth day after P.y 17XL infection. After 48 hours′ cultivation in vitro, the supernatant specimens of spleen cell culture in vitro were collected for detections of interferon-γ (IFN-γ) and tumor necrosis factor α (TNF-α) with enzyme-linked immunosorbent assay (ELISA).  Results  Compared with those of the normal control mice, the number of γδ T cells in spleen tissue of P.y 17XL infected mice increased significantly at the third and fifth day after the infection (both P < 0.05); while, the number of γδ T cells in spleen tissue of the mice with γδ T cell elimination decreased significantly (both P < 0.01). At the fifth day after the P.y 17XL infection, the number and activation of spleen DCs, the number of splenic Th1 cells, and the contents of IFN-γ and TNF-α in spleen cell culture supernatants increased significantly in mice with γδ T cell elimination in comparison with those in mice of P.y 17XL infection group.  Conclusion  γδ T cells are involved in acute phase infection of P.y 17XL in erythrocytic stage. After γδ T cell elimination, the Th1 immune response increases as a compensation.
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  • [1]
    Zhou QH, Wu FT, Pang LT, et al. Role of γδ T cells in liver diseases and its relationship with intestinal microbiota[J]. World Journal of Gastroenterology, 2020, 26(20): 2559 – 2569. doi: 10.3748/wjg.v26.i20.2559
    De Rosa SC, Andrus JP, Perfetto SP, et al. Ontogeny of gamma delta T cells in humans[J]. Journal of Immunology, 2004, 172(3): 1637 – 1645. doi: 10.4049/jimmunol.172.3.1637
    Dong P, Ju XW, Yan YW, et al. γδ T cells provide protective function in highly pathogenic avian H5N1 influenza a virus infection[J]. Frontiers in Immunology, 2018, 9: 2812. doi: 10.3389/fimmu.2018.02812
    Zhao YS, Lin L, Xiao ZG, et al. Protective role of γδ T cells in different pathogen infections and its potential clinical application[J]. Journal of Immunology Research, 2018, 2018: 5081634.
    Seixas EM, Langhorne J. γδ T cells contribute to control of chronic parasitemia in Plasmodium chabaudi infections in mice[J]. Journal of Immunology, 1999, 162(5): 2837 – 2841.
    Inoue S, Niikura M, Mineo S, et al. Roles of IFN-γ and γδ T cells in protective immunity against blood-stage malaria[J]. Frontiers in Immunology, 2013, 4: 258.
    Dieli F, Troye-Blomberg M, Farouk SE, et al. Biology of γδ T cells in tuberculosis and malaria[J]. Current Molecular Medicine, 2001, 1(4): 437 – 446. doi: 10.2174/1566524013363627
    Nakazawa S, Brown AE, Maeno Y, et al. Malaria-induced increase of splenic γδ T cells in humans, monkeys, and mice[J]. Experimental Parasitology, 1994, 79(3): 391 – 398. doi: 10.1006/expr.1994.1101
    Artavanis-Tsakonas K, Riley EM. Innate immune response to malaria: rapid induction of IFN-γ from human NK cells by live Plasmodium falciparum-infected erythrocytes[J]. Journal of Immunology, 2002, 169(6): 2956 – 2963. doi: 10.4049/jimmunol.169.6.2956
    Inoue SI, Niikura M, Takeo S, et al. Enhancement of dendritic cell activation via CD40 ligand-expressing γδ T cells is responsible for protective immunity to Plasmodium parasites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(30): 12129 – 12134. doi: 10.1073/pnas.1204480109
    Inoue SI, Niikura M, Asahi H, et al. γδ T cells modulate humoral immunity against Plasmodium berghei infection[J]. Immunology, 2018, 155(4): 519 – 532. doi: 10.1111/imm.12997
    Costa G, Loizon S, Guenot M, et al. Control of Plasmodium falciparum erythrocytic cycle: γδ T cells target the red blood cell-invasive merozoites[J]. Blood, 2011, 118(26): 6952 – 6962. doi: 10.1182/blood-2011-08-376111
    Mamedov MR, Scholzen A, Nair RV, et al. A macrophage colony-stimulating-factor-producing γδ T cell subset prevents malarial parasitemic recurrence[J]. Immunity, 2018, 48(2): 350 – 363. doi: 10.1016/j.immuni.2018.01.009
    Kemp K, Akanmori BD, Adabayeri V, et al. Cytokine production and apoptosis among T cells from patients under treatment for Plasmodium falciparum malaria[J]. Clinical and Experimental Immunology, 2002, 127(1): 151 – 157. doi: 10.1046/j.1365-2249.2002.01714.x
    Xu HB, Feng YH, Chen G, et al. L-arginine exacerbates experimental cerebral malaria by enhancing pro-inflammatory responses[J]. The Tohoku Journal of Experimental Medicine, 2015, 236(1): 21 – 31. doi: 10.1620/tjem.236.21
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