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Volume 38 Issue 5
May  2022
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LIU Jia-li, DING Gan-ling, WANG Jia-qi, . Antagonistic effect of andrographolide on cigarette smoke-induced lung injury in mice[J]. Chinese Journal of Public Health, 2022, 38(5): 585-588. doi: 10.11847/zgggws1136848
Citation: LIU Jia-li, DING Gan-ling, WANG Jia-qi, . Antagonistic effect of andrographolide on cigarette smoke-induced lung injury in mice[J]. Chinese Journal of Public Health, 2022, 38(5): 585-588. doi: 10.11847/zgggws1136848

Antagonistic effect of andrographolide on cigarette smoke-induced lung injury in mice

doi: 10.11847/zgggws1136848
  • Received Date: 2021-09-09
    Available Online: 2022-04-29
  • Publish Date: 2022-05-20
  •   Objective  To explore protective effect of andrographolide on lung injury in mice exposed to cigarette smoke, and to provide evidences for the prevention and treatment of chronic obstructive pulmonary disease (COPD).   Methods  Forty mice were randomly divided into a control group (A), cigarette smoke exposure group (B), normal saline group (C), and andrographolide group (D). All the mice were exposed to cigarette smoke (300 mg/m3) for one hour, twice a day, five days in a week for 12 weeks, except for those of group A. The mice of group D were treated with an intraperitoneal injection of andrographolide at dose of 20 mg/kg body weight once a week after last cigarette smoke exposure during the week and the mice of group C were treated simultaneously but with 200 μL saline. By the completion of all the treatments, lung tissue and blood samples of the mice were collected for determinations of alveolus mean lining interval (MLI), mean alveolar area (MAA), interleukin-6 (IL-6)/interleukin-8 (IL-8)/matrix metalloproteinase-9 (MMP-9) in serum and bronchoalveolar lavage fluid (BALF). Correlations among IL-8, IL-9 and MMP-9 were analyzed.   Results  The MLI, MAA, IL-6, IL-8 and MMP-9 of the mice exposed to cigarette smoke were significantly higher than those of the control mice. Compared with those of the mice with cigarette smoke exposure, the MLI, MAA, IL-6, IL-8 and MMP-9 were significantly decreased in the mice of andrographolide group. In BALF, the concentration of IL-6 and IL-8 were significantly correlated with that of MMP-9.   Conclusion  Andrographolide could reduce BALF MMP-9, exerting an anti-inflammatory effect on lung injury induced by cigarette smoke exposure in mice.
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  • [1]
    Adeloye D, Chua S, Lee C, et al. Global and regional estimates of COPD prevalence: systematic review and meta-analysis[J]. Journal of Global Health, 2015, 5(2): 020415. doi: 10.7189/jogh.05.020415
    [2]
    孟灿, 孙成昊, 商磊. PM2.5对慢性阻塞性肺疾病发生发展影响及药物治疗研究进展[J]. 中国公共卫生, 2019, 35(3): 377 – 380.
    [3]
    Chen ZM, Peto PR, Zhou MG, et al. Contrasting male and female trends in tobacco-attributed mortality in China: evidence from successive nationwide prospective cohort studies[J]. The Lancet, 2015, 386(10002): 1447 – 1456. doi: 10.1016/S0140-6736(15)00340-2
    [4]
    Hou W, Hu SY, Li CY, et al. Cigarette smoke induced lung barrier dysfunction, EMT, and tissue remodeling: a possible link between COPD and lung cancer[J]. BioMed Research International, 2019, 2019: 2025636.
    [5]
    Rovina N, Koutsoukou A, Koulouris NG. Inflammation and immune response in COPD: where do we stand?[J]. Mediators of Inflammation, 2013, 2013: 413735.
    [6]
    Labaki WW, Rosenberg SR. Chronic obstructive pulmonary disease[J]. Annals of Internal Medicine, 2020, 173(3): ITC17 – ITC32. doi: 10.7326/AITC202008040
    [7]
    Zhang QQ, Ding Y, Lei Y, et al. Andrographolide suppress tumor growth by inhibiting TLR4/NF-κB signaling activation in insulinoma[J]. International Journal of Biological Sciences, 2014, 10(4): 404 – 414. doi: 10.7150/ijbs.7723
    [8]
    何伊里, 何志义. 香烟烟雾暴露建立肺气肿小鼠骨骼肌并发症模型的方法[J]. 右江医学, 2012, 40(2): 190 – 193.
    [9]
    Botelho FM, Gaschler GJ, Kianpour S, et al. Innate immune processes are sufficient for driving cigarette smoke-induced inflammation in mice[J]. American Journal of Respiratory Cell and Molecular Biology, 2010, 42(4): 394 – 403. doi: 10.1165/rcmb.2008-0301OC
    [10]
    Bradford E, Jacobson S, Varasteh J, et al. The value of blood cytokines and chemokines in assessing COPD[J]. Respiratory Research, 2017, 18(1): 180. doi: 10.1186/s12931-017-0662-2
    [11]
    Liu X, Jones GW, Choy EH, et al. The biology behind interleukin-6 targeted interventions[J]. Current Opinion in Rheumatology, 2016, 28(2): 152 – 160. doi: 10.1097/BOR.0000000000000255
    [12]
    Nakamoto K, Watanabe M, Sada M, et al. Pseudomonas aeruginosa-derived flagellin stimulates IL-6 and IL-8 production in human bronchial epithelial cells: a potential mechanism for progression and exacerbation of COPD[J]. Experimental Lung Research, 2019, 45(8): 255 – 266. doi: 10.1080/01902148.2019.1665147
    [13]
    Ruwanpura SM, Mcleod L, Dousha LF, et al. Therapeutic targeting of the IL-6 trans-signaling/mTORC1 axis in pulmonary emphy-sema[J]. American Journal of Respiratory and Critical Care Medicine, 2016, 194(12): 1494 – 1505. doi: 10.1164/rccm.201512-2368OC
    [14]
    Quint JK, Wedzicha JA. The neutrophil in chronic obstructive pulmonary disease[J]. Journal of Allergy and Clinical Immunology, 2007, 119(5): 1065 – 1071. doi: 10.1016/j.jaci.2006.12.640
    [15]
    Løkke A, Lange P, Scharling H, et al. Developing COPD: a 25-year follow up study of the general population[J]. Thorax, 2006, 61(11): 935 – 939. doi: 10.1136/thx.2006.062802
    [16]
    Coffin CS, Osiowy C, Gao S, et al. Hepatitis B virus (HBV) variants fluctuate in paired plasma and peripheral blood mononuclear cells among patient cohorts during different chronic hepatitis B (CHB) disease phases[J]. Journal of Viral Hepatitis, 2015, 22(4): 416 – 426. doi: 10.1111/jvh.12308
    [17]
    Kennedy-Feitosa E, Cattani-Cavalieri I, Barroso MV, et al. Eucalyptol promotes lung repair in mice following cigarette smoke-induced emphysema[J]. Phytomedicine, 2019, 55: 70 – 79. doi: 10.1016/j.phymed.2018.08.012
    [18]
    Opdenakker G, Van Den Steen PE, Dubois B, et al. Gelatinase B functions as regulator and effector in leukocyte biology[J]. Journal of Leukocyte Biology, 2001, 69(6): 851 – 859.
    [19]
    Li YJ, Liu HT, Xu LX. Expression of MMP-9 in different degrees of chronic hepatitis B and its correlation with inflammation[J]. Experimental and Therapeutic Medicine, 2018, 16(5): 4136 – 4140.
    [20]
    Islam MT, Ali ES, Uddin SJ, et al. Andrographolide, a diterpene lactone from Andrographis paniculata and its therapeutic promises in cancer[J]. Cancer Letters, 2018, 420: 129 – 145. doi: 10.1016/j.canlet.2018.01.074
    [21]
    Guo WJ, Sun Y, Liu W, et al. Small molecule-driven mitophagy-mediated NLRP3 inflammasome inhibition is responsible for the prevention of colitis-associated cancer[J]. Autophagy, 2014, 10(6): 972 – 985. doi: 10.4161/auto.28374
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