Application of Random Amplified Polymorphic DNA(RAPD)in Molecular Epidemiological Studies of Vibrio Cholerae

Random amplified polymorphic DNA(RAPD)was established and was applied in molecular epiemiological studies of Vibrio cholerae.Two groups of primer sets were aimed at repetitive sequence of Vibrio cholerae,one group of primer was arbitrary sequence.2 O1 classical(CVC),81 EITor and 10 O139 strains were analysed wiht this mehtod.All strains have cholerae toxin gene and toxin coregulated pili gene with the detection of PCR.2 CVC,81 EVC and 10 O139 strains were divided into 2,14 and 3 RAPD types respectively.10 EVC strains isolated from one explosive event were divied into 2 RAPD types.It means that there were several infectious origins in this event.RAPD provides a rapid,simple and discriminable method in molecular epidemiological studies of Vibrio cholerae.