Detection of Serun Type by PCR and Necleotile Sequcnce of Viralstrains in Patients with Hemouuhagic Fever with Renal Syndrone in Baoding

To study Hantaan viral types in baoding Methods:RT Nested PCR was used to determinate Hantann viral(RNA and serum type)in serum samples from 31 acute patients with Hermorrhagic fever with renal syndrome.Some viral nedeic avid sequences were detected and compared with Hantaan viral standard strains reported athome and abrod.IN 31 acute patients with HFRS 24 serum samples were positire for seoul type:2 were poositive for Hantaan type.M gene of seoul type C3 strain was in 1985-2314 region.Compared with 76-118,A9 and LBY.the honologies of nucleic avid sequence of C3 strain were 68.79%,67.58%,68.03% respectively,amino avid sequence were 77.27%,77.27%,78.3% respectively.Compard with seoul and R22,the honologies OF nucleic avid sequence were 95.15%,93.94% respectively,amino avid aequences were 97.27%,97.27%.Compard with 76.118,A9.Seoul and R22,the homologies of LBY strain were 98.75%,87.15%,68.34%,68.03% respectively,amino and sequence were 100%,96.23%,78.3%,78.3%.Conclusion:It was certain firstly that was 76-118 subtype of Hantaan tye in Baoding and Hantaan type in Baoding.