Li Wenquan, . The Screen on Insland LEE Containing eae Gene from Special Fragment of EHEC O157∶H7[J]. Chinese Journal of Public Health, 2000, 16(12): 1097-1098. DOI: 10.11847/zgggws2000-16-12-24
Citation:
Li Wenquan, . The Screen on Insland LEE Containing eae Gene from Special Fragment of EHEC O157∶H7[J]. Chinese Journal of Public Health, 2000, 16(12): 1097-1098. DOI: 10.11847/zgggws2000-16-12-24
Li Wenquan, . The Screen on Insland LEE Containing eae Gene from Special Fragment of EHEC O157∶H7[J]. Chinese Journal of Public Health, 2000, 16(12): 1097-1098. DOI: 10.11847/zgggws2000-16-12-24
Citation:
Li Wenquan, . The Screen on Insland LEE Containing eae Gene from Special Fragment of EHEC O157∶H7[J]. Chinese Journal of Public Health, 2000, 16(12): 1097-1098. DOI: 10.11847/zgggws2000-16-12-24
The Screen on Insland LEE Containing eae Gene from Special Fragment of EHEC O157∶H7
To screen pathogenicity island LEE containing eae gene. Methods Special frament of EHEC O157:H7 was amplified by PCR and tagged as probe by DIG.Meanwhile,a fragment of LEE containing no SmaⅠsite was obtained through dot blothy-bridization and enzyme digestion.Result An about 23kb-long fragment of LEE was generated.Conclusion The lenghth of screened fragment is not in accordance with the predicted.
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