Dot Immunogold Filtration Assay for Detection of Antibody of Cysticercus in Patient Serum

To establish a more rapid and simple method of detecting antibody of cysticercus in patient serum.A dot immunogold filtration assay(DIGFA)was developed by coating the antig en of cysticercus on nitrocellulose(NC)membr ane,labeling staphylococcal protein A(SPA)with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 minutes.The coincidence rate of the results of simulataneous determination of serum with ELISA and DIGFA was 96% for ant ibody of cysticercus.DIGFA, more simple and rapid,is applicable to small medical laboratories. It could be applied for diagnosis and epidemiological inv estigation on cysticercosis.