Mechnism of Inhition of Ca2+ /CaM-PKⅡ after Combined Soman and Hypoxia Injury

ObjectiveTo study the mechanism of inhition of Ca²⁺/CaM-PKⅡ activity after combined soman and hypoxia injury.MethodsThe changes of the content of CaM and activity of Ca²⁺/CaM-PKⅡ were studied in brain tissue after combined soman and hypoxia injury with RIA for 12,24,48 hours.ResultsThe changes of the content of CaM were significantly higher at soman intoxicated group athypoxia than at soman intoxicated group and control group athypoxia;but the activity of Ca²⁺ /CaM-PKⅡ were significantly decreased;calcium antagonist Nimodipine,antagonized inhibition of Ca²⁺/CaM-PKⅡ activity.ConclusionCaM and Ca²⁺/CaM-PKⅡ lead to important role in the brain damage after combined soman and hypoxia injury.