Evaluation of Protective Effects of Fibroblast Growth Factor on Sulfur Mustard-induced Cytotoxicity

Objective To evaluate the protective effects of recombinant basic fibr oblast growth factor(rbFGF)on sulfur mustard(HD)induced cytotoxicity.Methods The HaCaT cell line derived from normal human epidermal keratinocytes was used as the research model.The protective effects of rbFGF on HD-induced cytotoxicity were evaluated by microscopic observation on morphologic change of cell culture,measurement of cell viability by XTT method and cellular membrane damage by flow cytometry(FCM).Results Compared with the HD-control group,the rbFGF treating group was found that:the number of floating cells was lesser while the number of cells sticking to the wall was more showed by microscopical examination; the OD value was significantly higher examined by XTT method;the M1 value(the percentage of PI fluor escing cells)was lesser showed by FCM.But 1 hour-pretr eatment of rbFGF failed to provide more prophylactic and ther apeutic effects.Conclusion rbFGF was found to provide significant protective effects against HD-induced cytotox icity in HaCaT cell line.