RT-PCR for Diagnosis of Rubella and Analysis on E1 Region DNA Sequencing

Objective To diagnose for rubella virus infection by aplication of RT-PCR method.Methods The rubella viral serum samples were collected from 3 regions in different times in Heilongjiang province.Those samples were IgM antibody positive by ELISA,and then were subjected to RT-PCR reaction for the amplification of E1 region.The RT-PCR amplifications were further sequenced and a phylogenetic tree was made on the basis of nucleotide sequences.Results Among those 10 samples of IgM antibody positive,4 samples were positive by RT-PCR reaction.In sequence analysis,three bp differenes were found between our 3 samples and the representative rubella virus strain of Thomas starin in UK and four bp differences were found between our 1 samples and the representative rubella virus strain of Thomas strain in UK.Conclusion Rubella virus E1 region was very stable and suitable to be as the RT-PCR amplification target for accurate and rapid diagnosis of rubella virus infection.