Effect of Levofloxacin Sulphonate on HBV RNA of 3.5kb and 2.1kb in 2.2.15 Cell Line

Objective To study the effect of levofloxacin sulphonate on HBV RNA of 3.5kb and 2.lkb in the 2.2.15 cell line.Methods HBV DNA,HBV RNA were examined by quantitative PCR;HBsAg and HBeAg were tested by radioimmunoassay.Results The quantity of HBV DNA,3.5kb HBV RNA and 2.1kb HBV RNA were 2.97pg/m1,0.40pg/ml and 0.27pg/ml respectively in the 2.2.15 cell line; 0.44pg/ml,0.20pg/ml and 0.13pg/ml respectively after treated by levofloxacin sulphonate(P<0.05 );the inhibited rate were 85.2%,50.0 0.6 and 48.1% respectively.The value of HBsAg and HBeAg were 42.1 and 36.0 respectively in the 2.2.15 cell superrant;6.0 and 16.2 respectively after treated by levofloxacin sulphonate,the inhibited rate were 85.7% and 55.6% respectively(P<0.05).Conclusion The effect of levofloxacin sulphonate against HBV infection may be through affecting the stability and translation of 3.5kb,2.1kb HBV RNA in the 2.2.15 cell line.