Polmorphisms of apoB Gene 3’ VNTR Alleles and Analysis of Lipid Level in Fishers

Objective To study the distribution and frequency of apoB gene 3' end alleles and try to find if there was any association with the level of plasma lipids.Methods PCR and gradient polyacrylamide gel electrophoresis were used to analysize the chromsome DNA and PCR products.Blood plasma lipids levels among different gene-type groups were also compared.Results Totally 10 alleles were found in the fishers group.The HVE32 and HVE34 were most common,and the frequencies of HVE32 and HVE34 were 44.33% and 33.6%,respectively.The apoC3 and apoC2 levels of VNTRBS group were lower than that of VNTRSS group,the apoB and apoC2 levels of heterozygote group were higher than that of homozygote group.Conclusion The characteristics of polymorphism apoB gene in fishers population included fewer gene type and lower frequency of big genotype,and so on.