Effects of Lovastatin on Proliferation Ability and pRb Protein Expression of MCF-7 Cells

ObjectiveTo investigate the mechanism of Lovastatin affecting the proliferation abilit y of MCF27 cells.MethodsThe growth curve,NBT reduction test,reverse transcription-PCR and western blotting analysis of Rb mRNA and protein were used in this study.ResultsLovastation could inhibit the cell proliferation and accelerate the differentiation of MCF27 cells.The expression of Rb mRNA was increased in Lovastation treated cells.Rb protein expression was gradually decreased in Lovastatin treated for 48-72h,the level of Rb protein expression was lower in all three doses groups than that in control group.ConclusionLovastatin may adjust the cell cycle processes and accelerate the differentiation through acting on Rb mRNA and protein expression in MCF27 cells.