Objective To understand the genetic type of Hantaviruses(HV)and the differences betw een the Msegments isolated from rodents in Shandong Province.
Methods The lung samples positive with HV antigens by immunofluorescent as say were amplified by RTPCR with Hantavirus specific primers and the products sequenced.The results were compared with the previous strains of Shandong Province in homolog y and phylogenetics.
Results Eighteen of the PCR amplifications were positive.Nucleotide sequence homology analysis showed that they were all seoul(SEO)-type HV.The nucleotide sequence homology of these samples was more than 95.7% and they belonged to the same subtype.The nucleotide sequence homology of the Hantaan(HTN)type HV in Shandong Province was more than 77.9%, but they belonged to different subtypes.
Conclusion The HV strains isolated in Shandong Province mainly were SEO type.The SEO type HV was highly conserved while the HTN type HV was highly variable.