Cloning and expression of Vitreoscilla Hemoglobin in Esherichia coli

  Objective   To construct one expression plasmid containing Vitreoscilla hemoglobin gene(vgb)-pBlurscript SK and to express vgb in E.cloi DH-5 A.

  Methods   Vitreoscilla came from our laboratory that selected from environment.Plasmid-pBlurscript SK was used vector.VHb gene was cloned by PCR technique.Recombinant expression plasmidpBlueV was constructed and was introduced into Escherichia.coli.The expression production of VHb in the host was ver ified by means of the CO-binding difference spectra and the SDS-PAGE and Western blot.

  Results   VHb gene molecular weight was 0.5 kb.DNA sequencing confirmed that the fragments in pBlue V was vgb gene.The homology was 100% compared with the published vgb gene fragments.

  Conclusion   Recombinant expression plasmid containing Vitreoscilla hemoglobin gene(vgb)was successfully constructed and expressioned in E.cloi.