Effects of manganese on activity of GAD in caudate putamen nucleus and substantia negra of filial rats

  Objective   To study the effects of manganese on the activit y of glutamic acid decarbox ylase(GAD)in the caudate putamen nucleus and substantia negra of filial rats in the pregnant rats exposed to mang anese.

  Methods   Pregnant rats were randomly divided into three groups, including group A, group Band group C, group Awas used as control group, group Band group Creceived intr aperitoneal injections of mang anese chloride(MnCl₂)38 mol/kg, 78 mol/kg respectivly for 8 times every two days.After normal delivery, the activity GADin the caudate putamen nucleus and Substantia Neg ra of 21 day old filial rata brains were deter mined with perox idase conjugated method.

  Results   The activity of GADin the Caudate putamen nucleus and Substantia Negr aof 21 day old rats of group Cwas increased significantly compared with group A(P < 0.01)and group B(0.01 < P < 0.05), and that of group Awas decreased significantly compared with group B(0.01 < P < 0.05).

  Conclusion   The activity of GADin caudate putamen nucleus and substantia Neg ra of br ains of filial rat could be promoted after the pregnant rats exposed to MnCl₂, so that the level of Glu was decreased and level of GABAwas increased in this area, and the ratio of Glu/GABAwas decreased, thus filial rats sremembrance was weaken.