Effects of Laurencia extract on antioxidant activities in mice

  Objective   The antiox idant activities of various dosages of Laurencia extract(LET)were investigated in mice.

  Methods   The total content of ter penoids in the LETwas detected by High Performance Liquid Chro matogr aphy (HPLC)and its mediallethaldose(LD₅₀)was estimated by Horn assay.The antiox idant activit ies of LETwere detected in mice.The oxidative DNA damage of peripher al lymphocytes induced by H₂O₂ was analyzed by SCGE.The concentrations of superoxide dismutase(SOD), malonyl dialehyde(MDA)in plasma were detected withe test kit.Data were analzyed by SPSS software.

  Results   The concentration of total ter penodis in the LET was proved to be 63.29%.The LETshowed lo wtoxicity with its LD₅₀ more than 3 160 mg/kg.There was a significant decreasing tendency of the oxidative DNA damage on peripheral lymphocytes in the 50 mg/kg supplement group induced by 10 mol/LH₂O₂.The lev els of MDA in the supplement groups were less than those of the control group.

  Conclusion   The LET rich in ter penoids is safety to be taken o rally.The 50 mg/kg LET can protect the peripherally mphocytes from oxidative DNA damage induced by H₂O₂.The LEThas anti lipid peroxidation activity.