Objective To select or optimize simple, fast and special means by compare of identification metheods after purification of Human herpesvirus-6B.
Methods The metheod of sucrose gradient centrifugation was used to purify Human herpesvirus-6B(HHV-6B); and in order to make sure the range of positive samples collection, the viron was detected by the metheods of PCR, Dot blotting, Western blotting.
Results Above metheods which all could detect the positive virus from samples, there were significant difference between the range of positive samples collection, time of identification and cost and so on.
Conclusion It is better to use the PCRand Dot blotting to primary identify the virus after HHV-6Bpurification.
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