Clone and expression of schistosoma japonicum p50 immunophilin gene and purification of p50 protein

  Objective   To clone and express Schistosoma japonicum(Sj)p 50 immunophilin gene, and to purify its protein.

  Methods   To amplify p 50 immunophilincs complete coding sequence(CDS)from adultworm mRNA by RT-PCR.cDNA of p 50 immunophilin gene was cloned into the pET30 a(+)vector.The recombinantplasmid was transformed into E.coli BL 21(DE3)and expressed, then the expressed products in E.coli were purified by affinity chromatography column.

  Results   p 50 immunophilin gene cDNA sequence was obtained and then successfully cloned into pET30 a(+)vector.p 50 immunophilin fusion protein was expressed afterinduced by IPTG and purified with(His)6 atthe Nterminus.

  Conclusion   Sjp 50 immunophilin gene was successfully cloned, expressed and fusion pr otein was purified, which gave the basis for further studyespecially as a vaccine.